Fluorescent Dye Phosphoramidites
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Fluorescein Phosphoramidites
Carboxyfluorescein (FAM) is the most used fluorescent dye for labeling oligonucleotides. FAM (in pair with BHQ-1) plays a particularly important role as a reporter in real-time PCR.
5-FAM Phosphoramidite
| Item | Cat. No | Pack | Price |
| 5-FAM Phosphoramidite CAS 147566-42-5 • C46H58N3O10P FW 843.9 • MC 552.5 ℹ️ Flexible linker. 6-FAM isomer is available on request. | 1101 | 100 mg | $100 |
| 200 mg | $130 | ||
| 1000 mg | $260 |
6-FAM Phosphoramidite
| Item | Cat. No | Pack | Price |
| 6-FAM Phosphoramidite CAS 952290-59-4 • C46H56N3O10P FW 841.9 • MC 535.4 ℹ️ Rigid linker. | 1103 | 100 mg | $100 |
| 200 mg | $130 | ||
| 1000 mg | $260 |
6-FAM Pro Phosphoramidite
| Item | Cat. No | Pack | Price |
| 6-FAM Pro Phosphoramidite CAS 959470-55-4 • C72H83N4O14P FW 1259.4 • MC 650.6 ℹ️ Pro linker allows internal labeling and label quantification. Also available as a nucleosidic phosphoramidite (100c) | 111e | 100 mg | $100 |
| 200 mg | $130 | ||
| 1000 mg | $260 |
JOE Phosphoramidites
4′,5′-Dichloro-2′,7′-dimethoxyfluorescein (JOE) is a fluorescein derivative used for labeling primers and qPCR probes (in pair with BHQ-1). It has emission maximum at 548 nm and allows taking advantage of the multicolor detection. We offer both 5-JOE and 6-JOE isomers as phosphoramidite reagents.
5-JOE Phosphoramidite
| Item | Cat. No | Pack | Price |
| 5-JOE Phosphoramidite CAS 1353002-88-6 • C52H64Cl2N3O12P FW 1025.0 • MC 664.1 ℹ️ Flexible linker. | 1115 | 100 mg | $200 |
| 200 mg | $290 | ||
| 1000 mg | $590 |
6-JOE Phosphoramidite
| Item | Cat. No | Pack | Price |
| 6-JOE Phosphoramidite CAS 1353002-87-5 • C52H62Cl2N3O12P FW 1023.0 • MC 662.0 ℹ️ Rigid linker was shown to be superior to the flexible linker, making quantum yield to be higher and less sequence dependent (J. Org. Chem., 2012, 77, 977. DOI). | 1114 | 100 mg | $200 |
| 200 mg | $290 | ||
| 1000 mg | $590 |
6-JOE Pro Phosphoramidite
| Item | Cat. No | Pack | Price |
| 6-JOE Pro Phosphoramidite C78H89Cl2N4O16P FW 1509.3 • MC 777.1 ℹ️ Pro linker allows internal labeling and label quantification. | 111f | 100 mg | $200 |
| 200 mg | $290 | ||
| 1000 mg | $590 |
TET Phosphoramidite
TET phosphoramidite contains a TET dye molecule (tetrachlorofluorescein) with absorption maximum at 522 nm and emission maximum at 538 nm. TET phosphoramidite can be used for labeling primers and qPCR probes (in pair with BHQ-1).
6-TET Pro Phosphoramidite
| Item | Cat. No | Pack | Price |
| 6-TET Pro Phosphoramidite C74H81Cl4N2O14P FW 1395.2 • MC 788.3 ℹ️ Pro linker allows internal labeling and label quantification. | 1129 | 100 mg | $100 |
| 200 mg | $170 | ||
| 1000 mg | $460 |
HEX Phosphoramidites
HEX phosphoramidites contain a HEX dye molecule (hexachlorofluorescein) with absorption maximum at 535 nm and emission maximum at 556 nm. HEX phosphoramidites can be used for labeling primers and qPCR probes (in pair with BHQ-1).
5-HEX Pro Phosphoramidite
| Item | Cat. No | Pack | Price |
| 5-HEX Pro Phosphoramidite C72H77Cl6N4O14P FW 1466.1 • MC 857.2 ℹ️ Pro linker allows internal labeling and label quantification. | 111c | 100 mg | $200 |
| 200 mg | $290 | ||
| 1000 mg | $590 |
6-HEX Pro Phosphoramidite
| Item | Cat. No | Pack | Price |
| 6-HEX Pro Phosphoramidite C72H77Cl6N4O14P FW 1466.1 • MC 857.2 ℹ️ Pro linker allows internal labeling and label quantification. Also available as a nucleosidic phosphoramidite (100d). | 112b | 100 mg | $200 |
| 200 mg | $290 | ||
| 1000 mg | $590 |
TAMRA Phosphoramidite
This phosphoramidite is based on a tetramethyl rhodamine dye with emission maximum at 580 nm. TAMRA plays an important role in both real-time PCR applications and as a FRET acceptor in pair with FAM. FAM is excited at 494 nm and transfers energy to TAMRA, which then emits light at 580 nm.
6-TAMRA Pro Phosphoramidite
| Item | Cat. No | Pack | Price |
| 6-TAMRA Pro Phosphoramidite C66H77N6O10P FW 1145.3 • MC 703.3 ℹ️ Pro linker allows internal labeling and label quantification. The reagent is readily soluble in acetonitrile. TAMRA is not stable under basic conditions and requires non-standard deprotection methods. | 111b | 100 mg | $200 |
| 200 mg | $330 | ||
| 1000 mg | $650 |
R6G Phosphoramidite
R6G phosphoramidite contains a rhodamine 6G dye with absorption maximum at 530 nm and emission maximum at 550 nm. R6G phosphoramidite can be used for labeling qPCR probes (in pair with BHQ-1).
6-R6G Phosphoramidite
| Item | Cat. No | Pack | Price |
| 6-R6G Phosphoramidite C46H52F6N5O8P FW 947.9 • MC 633.6 ℹ️ Rigid linker | 1124 | 100 mg | $200 |
| 200 mg | $290 | ||
| 1000 mg | $590 |
ROX Phosphoramidite
ROX phosphoramidite features the 6-carboxy-X-rhodamine (6-ROX) fluorophore, known for its strong red-orange emission (~600 nm), making it ideal for multiplexed assays where spectral separation is crucial. The Pro linker enables both 5′-end and internal modifications.
6-ROX Pro Phosphoramidite
| Item | Cat. No | Pack | Price |
| 6-ROX Pro Phosphoramidite C74H85N6O10P FW 1249.5 • MC 808.3 ℹ️ Pro linker allows internal labeling and label quantification. | 1147 | 50 mg | $400 |
| 200 mg | $1120 | ||
| 1000 mg | $3120 |
Cyanine Phosphoramidites
Cyanine phosphoramidites are based on four cyanine dyes: Cy3, Cy3.5, Cy5 and Cy5.5, all of which belong to the polymethine dye family. Cyanine phosphoramidites can be used for labeling primers and qPCR probes (Cy3 and Cy3.5 are usually paired with BHQ-2; Cy5 and Cy5.5 are usually paired with BHQ-3).
By-3 Pro Phosphoramidite
| Item | Cat. No | Pack | Price |
| By-3 Pro Phosphoramidite C65H81N5O6PBF4 FW 1146.2 • MC 617.3 ℹ️ Pro linker allows internal labeling and label quantification. Abs max 550 nm, em max 570 nm. | 1137 | 100 mg | $200 |
| 200 mg | $330 | ||
| 1000 mg | $650 |
By-5 Pro Phosphoramidite
| Item | Cat. No | Pack | Price |
| By-5 Pro Phosphoramidite C67H83N5O6PBF4 FW 1172.2 • MC 643.3 ℹ️ Pro linker allows internal labeling and label quantification. Abs max 649 nm, em max 670 nm. | 1139 | 100 mg | $200 |
| 200 mg | $330 | ||
| 1000 mg | $650 |
By-3.5 Pro Phosphoramidite
| Item | Cat. No | Pack | Price |
| By-3,5 Pro Phosphoramidite C76H91N5O6PBF4 FW 1288.4 • MC 759.4 ℹ️ Pro linker allows internal labeling and label quantification. Abs max 588 nm, em max 604 nm. | 1138 | 100 mg | $200 |
| 200 mg | $330 | ||
| 1000 mg | $650 |
By-5.5 Pro Phosphoramidite
| Item | Cat. No | Pack | Price |
| By-5.5 Pro Phosphoramidite C78H93N5O6PBF4 FW 1314.4 • MC 785.4 ℹ️ Pro linker allows internal labeling and label quantification. Abs max 675 nm, em max 694 nm. | 113a | 100 mg | $200 |
| 200 mg | $330 | ||
| 1000 mg | $650 |
