Reagents for Bioconjugation

Jump to:

FIDELITY OLIGOS LLC offers various reactive compounds for labeling, cross-linking and immobilization of biomolecules. These include activated esters (for amine labeling), azides and alkynes (for copper or metal-free click labeling), maleimides (for thiol labeling) as well as auxiliary reagents (buffers, catalysts or ligands) for bioconjugation.

As long as there are many possible combinations of specific fluorescent dyes or labels with reactive functional groups, we provide custom synthesis of the reagents. Please contact us for further information.

Activated esters

N-Hydroxysuccinimide (NHS) esters are widely used to label proteins, peptides or oligonucleotides bearing a reactive amine group. NHS esters are capable reacting with amines without carbodiimide activation.

6-FAM NHS

5-TAMRA NHS

6-TAMRA NHS

By-3 NHS

By-3.5 NHS

By-5 NHS

By-5.5 NHS

By-7-MO NHS

By-7-S NHS

Propynyloxypropyonic acid oxysuccinimide ester

γ-Azidobutyric acid oxysuccinimide ester

N-ε-Maleimidocaproyl oxysuccinimide ester

Alkyne-trebler-NHS

Biotin pentafluorophenyl ester

---

Azides

Azides are useful reagents for orthogonal bioconjugation. They react selectively with terminal alkynes in the presence of copper catalysts, which makes them valuable tools for bioconjugation and click chemistry (CuAAC) applications.

5-FAM Azide

5-FAM Azide, CHC protected

6-FAM Azide

6-FAM Azide C8

5-JOE Azide

5-JOE Azide, CHC protected

6-JOE Azide

5-HEX Azide

5-TAMRA Azide

6-TAMRA Azide

6-TAMRA Azide C8

5-R6G Azide

5-ROX Azide

6-ROX Azide

By-3 Azide

By-3.5 Azide

By-5 Azide

By-5.5 Azide

By-7 Azide

By-7-MO Azide

By-7-MO TEG Azide

By-7-S Azide

By-7-S TEG Azide

Coumarin 343 Azide

BBQ-650 Azide

Iowa Black FQ Azide

Iowa Black RQ Azide

Biotin Azide

Trebler GalNAc Azide

Imidazole-1-sulfonyl azide

---

Alkynes

Alkynes are useful reagents for orthogonal bioconjugation. They react selectively with azides in the presence of copper catalysts, which makes them valuable tools for bioconjugation and click chemistry applications.

5-FAM Alkyne

5-JOE Alkyne

5-JOE Alkyne, CHC protected

BBQ-650 Alkyne

Iowa Black FQ Alkyne

Iowa Black RQ Alkyne

---

Bicyclononynes (BCN)

Bicyclononyne group is a copper-free click chemistry (SPAAC) probe which enables highly efficient and completely orthogonal bioconjugation with various azide molecules. FIDELITY OLIGOS LLC offers single isomer BCN reagents (typically exo isomer) but alternative isomer can be available on request.

6-FAM BCN

5-JOE BCN

6-HEX BCN

6-TAMRA BCN

6-R6G BCN

Biotin BCN

Exo-BCN acid ethyl ester

Endo-BCN acid ethyl ester

---

Crosslinkers

In the context of bioconjugation and biomolecule modification, crosslinkers are often used to link biomolecules together, such as proteins or nucleic acids, or to attach biomolecules to surfaces or other materials. Heterobifunctional reagents allow obtaining bioconjugates of a target structure in good yields.

Alkyne maleinimide

Propynyloxypropyonic acid hydrazide

γ-Azidobutyric acid hydrazide

Propynyloxypropyonic acid oxysuccinimide ester

γ-Azidobutyric acid oxysuccinimide ester

N-ε-Maleimidocaproyl oxysuccinimide ester

---

Mass-tag reagents

Mass tag reagents, also known as mass spectrometry tags or mass spectrometry labels, are small molecules that are chemically attached to biomolecules to introduce a unique mass signature. These tags are used in mass spectrometry-based proteomics and metabolomics studies to label and track specific biomolecules, such as proteins, peptides, or metabolites, and facilitate their detection and quantification.

Deuterated methyl chloro­formate (d3-MCF, D-MCF)

Methyl chloroformate (MCF)

---

Trans-cyclooctenes (TCO)

Trans‑cyclooctenes (TCO) are highly strained alkenes that have become indispensable tools in modern bioconjugation because of their exceptional reactivity in inverse‑electron‑demand Diels–Alder reactions with tetrazines.

TCO NHS

TCO PEG4 NHS succinate

TCO TEG NHS carbonate

TCO TEG Phosphoramidite

TCO OH

---

Auxiliary reagents

Item	Cat. No	Pack	Price
TBTA (10 mM solution in DMSO) ℹ️ Click chemistry ligand for Cu(I) catalyst,	000a	1 mL	$40
		10×1 mL	$160

CuSO4 (10 mM solution in H2O) ℹ️ Click chemistry catalyst.	000b	1 mL	$40
		10×1 mL	$160

Sodium L-ascorbate CAS 134-03-2 • C6H8O6Na FW 176.1 ℹ️ Click chemistry reducing agent.	000c	2 mg	$40
		10×2 mg	$160

TEAA Buffer, 2M, pH 4÷11	000d	10 mL	$40
		10×10 mL	$160

Click Catalyst (10 mM solution) ℹ️ Cu2+/TBTA complex.	007b	1 mL	$30
		10×1 mL	$260

---

Graphene oxide

Graphene oxide (GO) is one of the most promising nanomaterials for various practical applications. In particular, it has great potential for the production of unique bioconjugates. We now offer GO in a form of stable colloidal solution in distilled water (may form easily dispersible precipitate over long storage) [1]. Sheet size is 30-500 nm (by Malvern Zetasizer Nano ZSP).

Graphene oxide