Nucleosidic Phosphoramidites
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5′-Amino Phosphoramidites
5′-Amino Phosphoramidites bearing a monomethoxytrityl protective group terminate an oligonucleotide with an amino group for further coupling or bioconjugation.
5′-MMTNH-dA(Bz) Phosphoramidite
| Item | Cat. No | Pack | Price |
| 5′-MMTNH-dA(Bz) Phosphoramidite CAS 184488-14-0 • C46H51N8O5P FW 826.9 • MC 312.2 | 102e | — | — |
| 200 mg | $140 | ||
| 1000 mg | $350 |
5′-MMTNH-dC(Ac) Phosphoramidite
| Item | Cat. No | Pack | Price |
| 5′-MMTNH-dC(Ac) Phosphoramidite CAS 125443-93-8 • C40H49N6O6P FW 740.8 • MC 288.2 | 102f | 100 mg | $300 |
| 200 mg | $400 | ||
| 1000 mg | $1130 |
5’-MMTNH-dG(iBu) Phosphoramidite
| Item | Cat. No | Pack | Price |
| 5’-MMTNH-dG(iBu) Phosphoramidite CAS 125443-93-8 • C40H49N6O6P FW 740.8 • MC 288.2 | 1030 | 100 mg | $300 |
| 200 mg | $400 | ||
| 1000 mg | $1130 |
5’-MMTNH-dT Phosphoramidite
| Item | Cat. No | Pack | Price |
| 5’-MMTNH-dT Phosphoramidite CAS 194034-71-4 • C39H48N5O6P FW 713.8 • MC 303.2 | 1031 | — | — |
| 200 mg | $230 | ||
| 1000 mg | $620 |
Oxopentyl Phosphoramidites
Oxopentyl-modified nucleoside phosphoramidites are designed for the synthesis of oligonucleotides containing a thermolabile 3′-blocking group, which prevents primer hybridization and extension at low temperatures and is automatically removed during high-temperature activation. Oligonucleotides synthesized using these phosphoramidites function as built-in hot-start primers, eliminating premature annealing and nonspecific extension during reaction setup and thermal cycling.
dA(Pac) Oxopentyl Phosphoramidite
| Item | Cat. No | Pack | Price |
| dA(Pac) Oxopentyl Phosphoramidite CAS 1097651-25-6 • C50H59N6O9P FW 919.0 • MC 399.3 ℹ️ Prepare 0.067M solution in dry MeCN. Cap A solution must be the phenoxyacetyl anhydride version of the reagent, not acetic anhydride. Deblock in 0.05M K2CO3 in MeOH, room temp, 16-20 h. | 1032 | — | — |
| 250 mg | $250 | ||
| 500 mg | $510 |
dG(Pac) Oxopentyl Phosphoramidite
| Item | Cat. No | Pack | Price |
| dG(Pac) Oxopentyl Phosphoramidite CAS 1097651-27-8 • C50H59N6O10P FW 935.0 • MC 415.3 ℹ️ Prepare 0.067M solution in mixture of dry dichloromethane : acetonitrile (1:1). Cap A solution must be the phenoxyacetyl anhydride version of the reagent, not acetic anhydride. Deblock in 0.05M K2CO3 in MeOH, room temp, 16-20 h. | 1033 | — | — |
| 250 mg | $250 | ||
| 500 mg | $510 |
dC(Ac) Oxopentyl Phosphoramidite
| Item | Cat. No | Pack | Price |
| dC(Ac) Oxopentyl Phosphoramidite C43H55N4O9P FW 802.9 • MC 375.3 ℹ️ Prepare 0.067M solution in dry MeCN. Cap A solution must be the phenoxyacetyl anhydride version of the reagent, not acetic anhydride. Deblock in 0.05M K2CO3 in MeOH, room temp, 16-20 h. | 1034 | — | — |
| 250 mg | $250 | ||
| 500 mg | $510 |
dT Oxopentyl Phosphoramidite
| Item | Cat. No | Pack | Price |
| dT Oxopentyl Phosphoramidite CAS 370596-61-5 • C42H54N3O9P FW 775.9 • MC 390.3 ℹ️ Prepare 0.067M solution in dry MeCN. Cap A solution must be the phenoxyacetyl anhydride version of the reagent, not acetic anhydride. Deblock in 0.05M K2CO3 in MeOH, room temp, 16-20 h. | 1035 | — | — |
| 250 mg | $250 | ||
| 500 mg | $510 |
Fluorescent Dye and Quencher Nucleosidic Phosphoramidites
Nucleosidic phosphoramidites are designed to introduce various labels into internal positions of the oligonucleotide during the automated synthesis. The resulting sequence retains its structure and base pairing ability. Also, the nucleosidic label does not affect the exonuclease or polymerase activity. FIDELITY OLIGOS LLC offers the most important nucleosidic phosphoramidites with fluorescent dyes or dye quenchers, alkyne anchor groups for further click conjugation, as well as amine, thiol and NHS functions.
6-FAM C6-dT Phosphoramidite
| Item | Cat. No | Pack | Price |
| 6-FAM C6-dT Phosphoramidite CAS 1194507-30-6 • C79H89N6O17P FW 1425.6 • MC 815.2 | 100c | 100 mg | $390 |
| 200 mg | $590 | ||
| 1000 mg | $1300 |
6-HEX C6-dT Phosphoramidite
| Item | Cat. No | Pack | Price |
| 6-HEX C6-dT Phosphoramidite C79H83Cl6N6O17P FW 1632.2 • MC 1019.0 | 100d | 100 mg | $460 |
| 200 mg | $650 | ||
| 1000 mg | $1560 |
BHQ-1 dT Phosphoramidite
| Item | Cat. No | Pack | Price |
| BHQ-1 dT Phosphoramidite C74H89N12O13P FW 1385.6 • MC 943.4 ℹ️ Quenching range 480–580 nm. | 100e | 100 mg | $590 |
| 200 mg | $900 | ||
| 1000 mg | $1950 |
Iowa Black FQ dT Phosphoramidite
| Item | Cat. No | Pack | Price |
| Iowa Black FQ dT Phosphoramidite C70H83N10O13P FW 1303.5 • MC 861.3 ℹ️ Quenching range 420–620 nm. | 1012 | 100 mg | $590 |
| 200 mg | $900 | ||
| 1000 mg | $1950 |
Iowa Black RQ dT Phosphoramidite
| Item | Cat. No | Pack | Price |
| Iowa Black RQ dT Phosphoramidite C71H79N8O13P FW 1283.4 • MC 841.3 ℹ️ Quenching range 500–700 nm. | 1013 | 100 mg | $590 |
| 200 mg | $900 | ||
| 1000 mg | $1950 |
Alkyne Nucleosidic Phosphoramidites
EdU Phosphoramidite
| Item | Cat. No | Pack | Price |
| EdU Phosphoramidite CAS 615288-66-9 • C41H47N4O8P FW 754.8 • MC 313.0 | 1014 | 100 mg | $390 |
| 200 mg | $590 | ||
| 1000 mg | $1300 |
5-Octadiynyl dU Phosphoramidite
| Item | Cat. No | Pack | Price |
| 5-Octadiynyl dU Phosphoramidite CAS 938186-76-6 • C47H55N4O8P FW 835.0 • MC 393.1 | 1015 | 100 mg | $390 |
| 200 mg | $590 | ||
| 1000 mg | $1300 |
6-Hexynoic acid alkyne C6-dT Phosphoramidite
| Item | Cat. No | Pack | Price |
| 6-Hexynoic acid alkyne C6-dT Phosphoramidite C54H69N6O10P FW 993.2 • MC 551.2 | 1016 | 100 mg | $390 |
| 200 mg | $590 | ||
| 1000 mg | $1300 |
Other Nucleosidic Phosphoramidites
S-Bz Thiol-Modifier C6-dT Phosphoramidite
| Item | Cat. No | Pack | Price |
| S-Bz Thiol-Modifier C6-dT Phosphoramidite C58H71N6O11PS FW 1091.3 • MC 545.2 | 100f | 100 mg | $390 |
| 200 mg | $590 | ||
| 1000 mg | $1300 |
Amino-Modifier C6-dT Phosphoramidite
| Item | Cat. No | Pack | Price |
| Amino-Modifier C6-dT Phosphoramidite CAS 210534-16-0 • C50H62F3N6O10P FW 995.0 • MC 457.2 | 100b | 100 mg | $390 |
| 200 mg | $590 | ||
| 1000 mg | $1300 |
NHS carboxy dT Phosphoramidite
| Item | Cat. No | Pack | Price |
| NHS carboxy dT Phosphoramidite CAS 1354695-41-2 • C46H52N5O12P FW 897.9 | 1017 | 100 mg | $390 |
| 200 mg | $590 | ||
| 1000 mg | $1300 |
RevdT Phosphoramidite
| Item | Cat. No | Pack | Price |
| RevdT Phosphoramidite CAS 134031-86-0 • C40H49N4O8P FW 744.8 • MC 303.0 | 100a | 100 mg | $90 |
| 200 mg | $130 | ||
| 1000 mg | $260 |
5′-POM-Vinyl phosphonate 2′-O-Methyl Uridine 3′-CE Phosphoramidite
| Item | Cat. No | Pack | Price |
| 5′-POM-Vinyl phosphonate 2′-O-Methyl Uridine 3′-CE Phosphoramidite CAS 1882067-09-5 • C32H52N4O13P2 FW 762.7 • MC 624.5 ℹ️ 5’ vinyl phosphonate mimics 5’-phosphorylation. | 1011 | 100 mg | $140 |
| 200 mg | $260 | ||
| 1000 mg | $1040 |
